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KMID : 0385219930030020102
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Korean Journal of Gerontology
1993 Volume.3 No. 2 p.102 ~ p.108
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Characterization of Microsomal Glutathione-Dependent Peroxidation Inhibiting Factor of the Rat liver
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Park Tae-Suk
Choi Kee-Oh
Wee Jung-Soon
Lee Min-Hwa
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Abstract
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This study was aimed to characterize further the glutathione-dependent peroxidation inhibiting factor (GPIF) which has recently been proposed by several investigators to act as a unique antioxidant in the rat liver microsomes. When microsomes were heat-denatured at 100℃ for 5 min, the GPIF activity disappeared completely, but the level of -tocopherol decreased only slightly. The GPIF activity was not detected in liposomes made of phospholipid and α-tocopherol. When microsomes containing 0.1 mM GSH were oxidized by meta-catalyzed oxidation system, GSH was consumed completely first, and then α-tocopherol began to be used. And lipid peroxidation began to occur after the α-tocopherol level was decreased to about 70%. These results suggest that microsomal GPIF may be an antioxidant protein that recycles tocopheroxyl radical to α-tocopherol in membranes, using GSH as a cosubstrate
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KEYWORD
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glutathione, tocopherol, lipid peroxidation, microsomes, antioxidant
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